| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2742 |
| 발표분야 |
생물화공 |
| 제목 |
Recombinant human G-CSF synthesized in E. coli BL21(DE3) suffers fromC-terminal degradation that is effectively prevented by N-terminal protein fusion |
| 초록 |
The solubility of human granulocyte colony-stimulating factor (hG-CSF) markedly increased in E. coli cytoplasm by using N-terminal fusion partner proteins. More importantly, we found that the C-terminal region of hG-CSF suffers from proteolytic degradation when hG-CSF is directly expressed in E. coli BL21(DE3). The recombinant hG-CSF that is expressed with an N-terminal fusion partner is effectively protected from the proteolysis. It seems that since the N-terminus of hG-CSF is located very close to the C-terminus, the presence of the N-terminal fusion partner masks the C-terminal region of hG-CSF and protects it from proteolytic degradation by E. coli protease(s). It is believed that rapid proteolysis occurs at the C-terminus of hG-CSF that is very easily exposed to E. coli protease(s) during a short period following protein synthesis and prior to completion of the formation of the inclusion body. |
| 저자 |
송종암, 한경연, 박진승, 서혁성, 안금영, 이종환, 이은정, 권수정, 신재욱, 김성은, 이지원
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| 소속 |
고려대 |
| 키워드 |
Human granulocyte colony-stimulating factor(hG-CSF); E. coli BL21(DE3); N-terminal fusion partner; C-terminal proteolysis
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| E-Mail |
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| 원문파일 |
초록 보기 |
