| 학회 | 한국공업화학회 |
| 학술대회 | 2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 | 25권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Enhanced production of isoprenoids through precursor rebalancing strategy in Escherichia coli |
| 초록 | Due to their broad applicability, various attempts for isoprenoids biosynthesis have been held. As one of the major isoprenoid production pathways, the 1-deoxy-D-xylulose-5-phosphate (DXP) pathway, requires equimolar glyceraldehyde 3-phosphate and pyruvate, it is crucial achieving a proper diversion of carbon flux for the optimal production in Escherichia coli. Here, we introduce a fine-controlled precursor rebalancing strategy regulating gapA expression level. As a model system, a lycopene-overproducing E. coli strain was constructed by sequential amplification of the DXP pathway using synthetic expression cassettes. Then, we investigated the effect of precursor balancing with PpsA and GAPDH through fine-tuning at both transcription and translation levels. As a consequence, the down-regulation of gapA improved the specific lycopene content by 45% compared to the overexpression of ppsA, suggesting gapA as a good target for precursor balancing to improve isoprenoids biosynthesis. |
| 저자 | 김동환, 정주영, 임재형, 김세연, 석주연, 정규열 |
| 소속 | 포항공과대 |
| 키워드 | Precursor balancing; Isoprenoid; Lycopene; gapA; ppsA; DXP pathway |
