| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Synthetic biosensor-based evolutionary engineering of aldehyde dehydrogenase for efficient 3-hydroxypropionic acid production |
| 초록 |
3-Hydroxypropionic acid (3-HP) is an platform chemical, and biological production of 3-HP from glycerol has been revealed to be effective because of a simple pathway, higher yield and productivity. Despite the successful 3-HP production from glycerol, the biological process suffers from problems of low activity and inactivation of the two enzymes. To apply the directed evolutionary approach to engineer the 3-HP production system, we constructed a synthetic biosensor using a 3-HP responsive transcription factor for screening of 3-HP high-producing mutant. The method was applied to an ALDH library, specifically aldehyde-binding site library of α-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency than the wild-type. Our approach provides a simple and efficient tool for evolutionary protein engineering. |
| 저자 |
민명원1, 석주연1, 임현규2, 박성훈3, 유태현4, 정규열1
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| 소속 |
1포항공과대, 2UCSD, 3UNIST, 4아주대 |
| 키워드 |
synthetic biosensor; evolutionary protein engineering; 3-hydroxypropionic acid
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| E-Mail |
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