| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Directed Evolution of Aldehyde Dehydrogenase for improving the Catalytic Efficiency toward 3-HPA via 3-HP Selection Device and 96-well microplate-based Screening |
| 초록 |
3-Hydroxypropionic acid (3-HP) is one of the key building blocks which is a precursor of diverse valuable chemicals. The molecule can be produced by a simple biological pathway: dehydration of glycerol to 3-hydroxypropanal (3-HPA) by glycerol dehydratase and then oxidation to 3-HP by aldehyde dehydrogenase. Despite successful production of 3-HP through the pathway, several problems including the toxicity of the 3-HPA hamper the 3-HP production by industrial level. To solve the issues, we further engineered α-ketoglutaric semialdehyde dehydrogenase from Azospirilum brasilense for enhancing the catalytic activity toward 3-HPA via transcriptional factor-based selection device and 96well microplate-based screening based on the new big library (3.3*106) which includes 5 residues. Finally, we got the final mutant which has 4.65-fold higher activity than WT. Nowadays, we are going to conduct several analyzes including MD simulations and 3-HP production. |
| 저자 |
박예섭1, 유태현1, Nasir Abdul1, 정규열2, 석주연2
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| 소속 |
1아주대, 2포항공과대 |
| 키워드 |
Enzyme engineeirng; 3-HP
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| E-Mail |
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