| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Gene delivery with a non-viral carrier to adult stem cells |
| 초록 |
In this study, we evaluated the transfection efficiency of polyethyleneimine (PEI) as a gene carrier in two kinds of adult stem cells—rat bone marrow mesenchymal stem cells (rBMSCs) and rat muscle-derived stem cells (rMDSCs). The results of agarose gel electrophoresis identified that DNA is fully bound to PEI at the N/P charge ratio 4. The surface charge of the DNA/PEI nanoparticles was positive at the N/P charge ratio greater than 4. According to the results of cytotoxicity assay, the viability of rBMSCs and rMDSCs was inversely proportional to the N/P charge ratio of DNA/PEI nanoparticles. The fluorescence imaging of cells showed EGFP expression clearly in both kinds of stem cells transfected with DNA/PEI nanoparticles. More specifically, rBMSCs showed high transfection efficiency (1-17%) whereas rMDSCs showed relatively lower efficiency (1-5%). Our results demonstrate that rBMSCs were more effectively transfected with DNA/PEI nanoparticles than were rMDSCs. |
| 저자 |
김령미, 김다연, 권진선, 손아름, 김은화, 김재호, 김문석
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| 소속 |
아주대 |
| 키워드 |
bone marrow stem cells; muscle-derived stem cells; polyethyleneimine; gene delivery
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| E-Mail |
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