| 초록 |
The promoter activity is a key factorin a cellfor the activegrowth of living things and mass production of useful materials. To investigate the promoter activity of eukaryotic cell, in-vivo transcription has been usually used.However, for the faster monitoring of genetic information processing, in-vitro transcription process is essentiallyneeded, becauseeukaryotic cellgrows up slowly.Here we compared the several promoters activities by a simplified microalgal in-vitro transcription. RNA polymerase and the other cofactors were seperated from the chlamydomonas's cell lysate using MNP-DNA conjugates. Subsequently, concentration of mRNAproduced by in-vitro transcription was measured by Qubit fluorometer. As a results, wecould observe the changes in amounts of mRNA depends on the kinds of promoters. Thus, the best promoter could be selected.This system allows the fast, easy and economical analysis of promoter activity of microalgal under in-vitro transcription condition. |
