| 초록 |
The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. The promoter activity is a key factor in a cell for the active growth of living things and mass production of useful materials, but also abnormal growth of cancer cells. To investigate the promoter activity, interactions of RNA polymerase with their promoters have also been studied by a variety of biochemical methods, like an in-vivo and in-vitro transcription. Using SP6 promoter-RNA polymerase as a model, different LSPR λmax shift were recorded with various promoter sequences induced by point mutation. This result successfully demonstrates a non labeling and simple analysis for promoter activity, and it possesses a great potential as a sensitive, on-chip and multiplexing gene analysis. |
