| 초록 |
In the present study, we focused on developing a variety of hazardous biomarkers of specific expression in medaka exposed to the ecotoxic chemical. Recent developments in molecular biology offer new possibilities for developing biomarker with the least of false positive. In this work, a new differential display-PCR (DD-PCR) method based on annealing control primers (ACPs) was investigated in order to develop hazardous biomarkers from differentially expressed genes (DEGs) of mdeaka (Oryzias latipes) in the exposed to diazinon and in the unexposed areas. As a result, we identified 83 DEGs from medaka treated with diazinon. Among these genes, ribosomal protein genes related cancer were quantified by real-time PCR, we identified potential possibility as hazardous biomarkers in aquatic ecosystem. |
