| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1743 |
| 발표분야 |
생물화공 |
| 제목 |
Development of Specifically Aptamer Binding to Capnocytophaga ochracea a Causing of a Periodontitis |
| 초록 |
Capnocytophaga ochracea is a Gram negative bacillus which grow in atmosphere that has at least 5% CO₂ due to the it’s total dependence of fixing CO₂. It exists as a part of the normal flora in human oral periodontal tissue and can cause disease periodontitis. The original method of diagnose was to extract the blood of patients and identify the infection of the strain. In this study, we have developed single-stranded DNA aptamers to detect C.ochracea. The aptamers have the advantages such as stable tertiary structure, small size and steady activation. The aptamer is able to bind receptors or proteins located on the cell surface of bacteria with high affinity and specificity, and to detect bacteria quickly and easily. The aptamer that binds to the target C.ochracea, we utilized SELEX (Systematic Evolution of Ligands by exponential Enrichment) process to find. Currently we are at the process of selecting ssDNA binding to the target. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012653)” Rural Development Administration Republic of Korea. |
| 저자 |
우주아1, 신우리1, Simranjeet Singh Sekhon1, 안지영1, 조성진1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
