| 초록 |
BSA has numerous biochemical applications including stabilize some enzymes during digestion of DNA to prevent adhesion of the reaction tune, pipet tips and other vessels. And also often used as a protein concentration standard in lab experiments. We studied to the complementarities of two techniques, dynamic light scattering(DLS) and static light scattering detector coupled with a size exclusion chromatography system(SEC-LS), using denatured and aggregated BSA have been dosed at different levels into a non-denatured sample. The SEC-LS result shows that content of the aggregator and each molecular weight. The DLS typically changed the total intensity of scattered light by sample, non-denatured particle has 5nm but can be checked generated aggregation peak over time. By comparing the SEC-LS and DLS, it is complementary techniques to determine the amount of aggregator and identifying individual molecules. |
