| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Efficient Local Gene Transfer in Normal and Infarct Hearts mediated by Thermo-Sensitive and Biodegradable Polymeric Hydrogel |
| 초록 |
Thermo-sensitive and biodegradable polymeric hydrogel has been synthesized and investigated for local gene transfer in heart in order to recover cardiac infarction. Initially, luciferase gene was delivered to heart in mouse to test the duration and intensity of gene expression. Gene expression intensity assessed by optical imaging is closely correlated to actual expressed protein measured by luciferase assay. Polymeric hydrogel based gene transfer was shown to mediate enhanced gene expression up to 4 fold, compared to naked plasmid, and have two expression profiles after local injection. Histologic analyses has revealed that high gene expression is initially dominated by myocardium, whereas lower and longer expression is mainly governed by fibrotic and/or inflammatory cells infiltrated into injury site during injection. Significant expression was also noticed with lucifease gene in hydrogel after injecting in infarcted site of rat myocardial infarction model. |
| 저자 |
박인규1, 권진숙2, 민정준3, 정명호2, 김원종4, 조상봉5, Suzie H. Pun6, 안영근7
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| 소속 |
1전남의대 의생명과학과 / GIST 바이오광학영상센터, 2전남의대 순환기내과, 3전남의대 핵의학과, 4포항공대 화학과, 5Dept. of Phamaceutics, 6The Univ. of Mississippi, 7Department of Bioengineering |
| 키워드 |
Thermosensitive; cardiac infarction; bioluminescence optical imaging; gene therapy
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| E-Mail |
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