| 학회 |
한국고분자학회 |
| 학술대회 |
2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
41권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Cross-linked Hyaluronic Acid with 3D-printed Porous Polylactide Scaffolds for 3D Cell Culture |
| 초록 |
Three dimensional (3D) cell culture systems, which mimic a cell’s in situ environment in tissues, have great potential for overcoming the inherited limitation of two dimensional cultures. Here we introduce a cell-loaded hyaluronic acid (HA) hydrogel network cross-linked via catechol-ferric ion complexation within mechanically stable, open porous polylactide (PLA) scaffolds for 3D tissue culture. 3D PLA scaffolds were fabricated by a fused-deposition-modeling 3D printing technique. HA was chemically grafted with catechol groups and injected into the PLA scaffolds that were pre-treated with ferric chloride to generate a cross-linking network of HA. The loading efficiency of HeLa cancer cells was 89.2 % without significant cytotoxicity. We expect that our approach to generate a 3D hybridized structure will provide a promising materials platform for 3D culture of various tissues. Acknowledgement: Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1402-10. |
| 저자 |
안재환, 남윤성
|
| 소속 |
한국과학기술원 |
| 키워드 |
3D culture;; 3D printing; polylactide; hyaluronic acid; catechol
|
| E-Mail |
|
