| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Development of a protease assay method via engineering of procaspase-3 enzymes |
| 초록 |
Proteases are implicated in an essential role of the biological processes including diseases development. In this study, a novel activity assay method for matrix metalloproteinases is established by using an engineered procaspase-3 by replacement of cleavage site in the procaspase-3 to recognize by interest protease. As a result, the engineered pro-caspase-3 can be purely activated by MMP-2 and the enzyme activity was increased 225.9-fold after proteolysis. The assay could be distinguished pico-molar range of MMP-2. By development of the high sensitive and simple assay method for protease activity, the assay is expected to be applied diagnosis for MMP2-associated diseases and easily adapted for other proteases. |
| 저자 |
박현지1, 양도경2, 유태현1
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| 소속 |
1Department of Molecular Science and Technology, 2Ajou Univ. |
| 키워드 |
Protease activity assa; MMP2; zymogen; enzymatic cascade
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| E-Mail |
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