| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
Label free analysis of antibody-antigen binding using bioresponsive hydrogels and SPR |
| 초록 |
Antibodies are composed of two identical antigen binding sites and can form ternary complexes with proteins through divalent binding. The precise identification of antigen-antibody binding kinetics is expected to help in the discovery and development of new drugs and treatments. However, there is still no efficient and label free method for the precise identification of these kinetics. Here, we report a label-free hydrogel-based SPR sensor for the quick determination of antibody-antigen binding and its kinetics. We were able to evaluate antibody-antigen binding events using a SPR chip modified with bioresponsive hydrogels. With our method, we were able to observe a 2 to 3-fold enhancement in SPR signal intensity for multivalent antibody-antigen binding. Also, we were able to measure the binding kinetics of PD-1 IgG antibody at 10mM with a SNR value above 2. |
| 저자 |
Teoh Jie Ying, 유동원, 임국희, 양해민, 전수환
|
| 소속 |
서울대 |
| 키워드 |
hydrogels; antibody-antigen binding; label free; surface plasmon resonance; biosensor
|
| E-Mail |
|
