학회 |
한국화학공학회 |
학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
권호 |
24권 2호, p.2127 |
발표분야 |
[C1가스리파이너리사업단 국제 심포지엄] 바이오촉매관련(생물화공부문위원회) |
제목 |
Hexanoic acid production from CO by Clostridium sp. JS66: physiological and metabolic characterization |
초록 |
C1 gases including carbon monoxide, carbon dioxide, and hydrogen are emitted from steel industrial facilities, causing global warming. C1 gases are recently spotlighted as feedstocks for fuels/chemicals production. Especially, gas fermentation by acetogenic bacteria utilizing C1 gases as carbon and energy sources has been studied for converting C1 gases to valuable biofuel/chemicals. Here, we report a newly isolated strain Clostridium sp. JS66 from mudflat as a hexanoic acid producer from C1 gases. Clostridium sp. JS66 exhibited over 99% of 16s rRNA similarity to C. carboxidivorans P7, a known hexanoic acid-producing acetogen; but, distinct phenotypic differences, an average nucleotide identity (ANI) value of 95.5 %, and DNA-DNA hybridization value of 33 % suggests that Clostridium sp. JS66 is a noble species distinct from C. carboxidivorans P7. Further physiological and metabolic characterization was performed through optimizing fermentation conditions, analyzing genomic data, and identifying key pathways for hexanoic acid production. |
저자 |
엄영순1, 김중석1, 김재현1, 김경식1, 김정연2, 김경헌2
|
소속 |
1KIST, 2고려대 |
키워드 |
Carbon monoxide; steel waste gas; acetogen; hexanoic acid
|
E-Mail |
|
원문파일 |
초록 보기 |