화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2018년 가을 (10/24 ~ 10/26, 대구 EXCO)
권호 24권 2호, p.2127
발표분야 [C1가스리파이너리사업단 국제 심포지엄] 바이오촉매관련(생물화공부문위원회)
제목 Hexanoic acid production from CO by Clostridium sp. JS66: physiological and metabolic characterization
초록 C1 gases including carbon monoxide, carbon dioxide, and hydrogen are emitted from steel industrial facilities, causing global warming. C1 gases are recently spotlighted as feedstocks for fuels/chemicals production. Especially, gas fermentation by acetogenic bacteria utilizing C1 gases as carbon and energy sources has been studied for converting C1 gases to valuable biofuel/chemicals. Here, we report a newly isolated strain Clostridium sp. JS66 from mudflat as a hexanoic acid producer from C1 gases. Clostridium sp. JS66 exhibited over 99% of 16s rRNA similarity to C. carboxidivorans P7, a known hexanoic acid-producing acetogen; but, distinct phenotypic differences, an average nucleotide identity (ANI) value of 95.5 %, and DNA-DNA hybridization value of 33 % suggests that Clostridium sp. JS66 is a noble species distinct from C. carboxidivorans P7. Further physiological and metabolic characterization was performed through optimizing fermentation conditions, analyzing genomic data, and identifying key pathways for hexanoic acid production.
저자 엄영순1, 김중석1, 김재현1, 김경식1, 김정연2, 김경헌2
소속 1KIST, 2고려대
키워드 Carbon monoxide; steel waste gas; acetogen; hexanoic acid
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