| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
[생물공학] 팬데믹 이후 및 4차산업 시대의 바이오테크놀로지 |
| 제목 |
Automated strain development using robotics and CRISPR genome engineering |
| 초록 |
A biofoundry provides an integrated robotic platform to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications. I present here the SKy Biofoundry to perform automated strain development using robotics and CRISPR genome engineering. The SKy Biofoundry is capable of performing automatic actions for various molecular and microbiological experiments such as gene cloning and colony selections using an integrated robotic and CRISPR-genetic platform. To facilitate the power of the automated platform, high fidelity CRISPR-base editing system was developed to create a sequence-specific cytosine conversion without the off-target mutations. The SKy Biofoundry has been hosting to construct massive genetic variants of Corynebacterium glutamicum and Escherichia coli using the CRISPR-Cas system to promote automated strain development in the Design-Build-Test-Learn cycle. |
| 저자 |
우한민 |
| 소속 |
성균관대 |
| 키워드 |
Biofoundry; CRISPR; genome engineering
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| E-Mail |
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