| 초록 |
We grafted acrylic acid (AAc), acrylamide (AAm), collagen to PLGA to enhance hepatocyte adhesion. The adhesion of hepatocyte was determined by plating 1´105 cell/ml (24 well plate) and quantifying percentage of adhered cells after 4, 8, 12, 24 h. Hepatocytes were plated 1´104 cell/ml and were incubated for 1, 3, 4 day. The cell morphology was observed by microscopy. Albumin and CK18 were confirmed to exist in hepatocyte by western blot analysis and immunofluorescent staining. And hepatocyte functional activity, including glycogen synthesis, and urea production were confirmed by periodic acid Shiff (PAS) staining and urea assay. The adhesion of hepatocyte number on modified PLGAs was more than that on PLGA. And we could see that a slight accumulation of glycogen was detected. Urea production of cultured hepatocyte on modified PLGA was increased. Therefore the hepatocytes showed the best maintenance of albumin secretion and urea synthesis functions than those cultured on PLGA-collagen scaffold. These results indicated that modified PLGA scaffold with AAc, AAm, and collagen were found to enhance hepatocyte adherence and function. |
