| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Biosynthesis of Nylon 12 monomer, ω-aminododecanoic acid using artificial self-sufficient P450, Alcohol dehydrogenase and w-Transaminases. |
| 초록 |
ω-Aminododecanoic acid is considered as one of the potential monomers of Nylon 12, a high-performance member of bioplastic family. The biosynthesis of ω-aminododecanoic acid from renewable sources is an attractive process in the polymer industry. Here, we constructed three artificial self-sufficient P450s using cytochrome P450 reductase (CPR) domains of natural self-sufficient P450s. Among them, artificial self-sufficient P450 (CYP153A13BM3CPR) with the CYP102A1 CPR showed the highest catalytically activity for dodecanoic acid (DDA) substrate. This form of ArtssP450 was further co-expressed with ω-Transaminase and alcohol dehydrogenases. This single-cell system was used for the biotransformation of DDA to ω-aminododecanoic acid (ω-AmDDA), wherein we could successfully biosynthesize 1.48 mM ω-AmDDA from 10 mM DDA substrate in the one-pot reaction. |
| 저자 |
성시형1, 김건희1, 유소정1, Mahesh D. Patil1, Ahsan MM2, 윤형돈1
|
| 소속 |
1건국대, 2영남대 |
| 키워드 |
artificial self-sufficient P450; bioplastics; dodecanoic acid; Nylon 12; ω-aminododecanoic acid
|
| E-Mail |
|
