| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 가을 (10/27 ~ 10/29, 광주 김대중컨벤션센터) |
| 권호 |
27권 2호, p.1700 |
| 발표분야 |
생물화공 |
| 제목 |
Efficient 3-hydroxypropionic acid production by biosensor-based evolutionary engineering of aldehyde dehydrogenase |
| 초록 |
3-Hydroxypropionic acid (3-HP) is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been revealed to be effective because of simple pathway and higher yield and productivity. However, 3-HP production from glycerol have problem of low activity and inactivation of GDHt and ALDH. For evolutionary protein engineering, 3-HP responsive transcription factor based biosensor was constructed and applied to an ALDH library, specifically aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type one. Our approach provides the simple and efficient tool to engineer the pathway enzymes in metabolic engineering. |
| 저자 |
민명원1, 석주연1, 임현규1, 박성훈2, 유태현3, 정규열1
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| 소속 |
1POSTECH, 2UNIST, 3아주대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
