| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
[생물공학] 바이오팩토리 합성 최신현황과 차세대응용 |
| 제목 |
Development of assay methods based on proximity proteolysis reaction |
| 초록 |
Various types of molecules serve as biomarkers of diseases, and numerous methods have been reported to detect and quantify them. Recently, research efforts have been made to develop point-of-care (POC) tests, which contribute to early diagnoses of diseases, particularly in resource-limited settings. An assay performed in a homogeneous phase is an obvious route to develop these methods. Here, simple homogeneous methods based on proximity proteolysis reactions (PPR) are reported to detect biological molecules. A typical PPR system has been designed such that the proteolysis reaction between protease and zymogen is enhanced in the presence of a target analyte. The activated zymogen generates a color signal by hydrolyzing a chromophore. The modular design of the PPR-based assay will enable the development of simple POC diagnostics for various biomarkers. |
| 저자 |
유태현 |
| 소속 |
아주대 |
| 키워드 |
zymogen; protease; proximity; assay
|
| E-Mail |
|
