| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
High-throughput screening of aldehyde dehydrogenase for 3-hydroxypropionic acid production using an in vivo synthetic selection device |
| 초록 |
3-HP is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase and aldehyde dehydrogenase has been revealed to be effective. Despite successful attempts of 3-HP production, biological process suffers from problems arising from low activity and inactivation of the two enzymes. To apply the directed evolution to engineer 3-HP production system, we constructed a synthetic screening device using a 3-HP-responsive transcription factor and developed a selection approach for screening. The method was applied to ALDH library, specifically aldehyde-binding site library of KGSADH. Only two serial cultures resulted in enrichment of strains showing 25% increased 3-HP production, and an isolated KGSADH variant enzyme exhibited 2.8-fold higher catalytic efficiency toward its aldehyde substrate than wild-type one. |
| 저자 |
백동엽1, 석주연1, 양진아2, 박성훈3, 유태현4, 정규열1
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| 소속 |
1POSTECH, 2제주대, 3UNIST, 4아주대 |
| 키워드 |
3-Hydroxypropionic acid; glycerol; Escherichia coli; High-throughput screening
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| E-Mail |
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