| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 봄 (04/22 ~ 04/23, 여수대학교) |
| 권호 |
11권 1호, p.367 |
| 발표분야 |
생물화공 |
| 제목 |
Analysis of NADPH regeneration systems in recombinant Saccharomyces cerevisiae for production of xylitol from xylose |
| 초록 |
Xylitol has been used as a sugar substitute in the food industry due to a number of functional properties such as low caloric and anti-cariogenic characteristics. As NADPH plays an important role in xylitol biosynthesis from xylose in recombinant Saccharomyces cerevisiae containing the xylose reductase (XR) gene from Pichia stipitis, the NADPH regeneration system is required for effective production of xylitol. NADPH is mainly generated through the oxidative part of the pentose phosphate pathway by the action of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) expressed by the ZWF1 and GND1 genes, respectively, in S. cerevisiae. To facilitate NADPH regeneration, the ZWF1 gene was expressed in recombinant S. cerevisiae harboring the XR gene from P. stipitis. Its effect on xylitol production was investigated in batch and glucose-limited fed-batch fermentations. In batch fermentations, xylitol productivity for S. cerevisiae BJ3505:δXR/pKZWF1 resulted in 1.7 g/L•hr, which was about 1.2-fold higher than that obtained from the control strain (1.4 g/L•hr). Glucose limited fed-batch fermentation was performed for sufficient supply of ATP as a maintenance energy and NAD(P)H as a reducing power for xylitol production. Overexpression of the ZWF1 gene improved xylitol productivity from 1.7 g/L•hr to 2.3 g/L•hr in fed-batch fermentation of recombinant S. cerevisiae. |
| 저자 |
권도현, 이태희, 김희정, 김정아, 유현아, 이원흥, 서진호
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| 소속 |
서울대 |
| 키워드 |
Xylitol; Saccharomyces cerevisiae; Xylose reductase; NADPH; Glucose-6-phosphate dehydrogenase; 6-phosphogluconate dehydrogenase; Glucose-limited fed-batch fermentation
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| E-Mail |
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| 원문파일 |
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