| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
고분자 가공/복합재료 |
| 제목 |
Green Fluorescent Protein Adsorption with Metal Organic Framework-coated Quartz Crystal Microbalance Plate |
| 초록 |
Interaction of Oligohistidine-tags(His-tag) with metal ions have long have been known and used for various purposes. Here, we report a method for attaching His-tagged proteins to a Metal Organic Framework(MOF)-coated Quartz Crystal Microbalance(QCM) plate. QCM is capable of detecting nanogram-scale mass change with a simple procedure, giving immediate quantitative results. Especially, MOF-coated QCM is predicted to have increased efficiency in mass-change sensitivity. In this research, we selected MOF candidates with metal ions that are known to interact strongly with his-tags and coated them on the QCM plate. For determining the interaction with his-tagged proteins, we applied His-tagged Green Fluorescent Protein(GFP) to the MOF film and observed the color change with a UV microscope, having successful results. This opens a new possibility for using His-tagged protein attached MOF QCM as a tunable sensor, depending on what receptor we utilize as a protein. |
| 저자 |
박우진1, 김태훈1, 이준성1, 유호연2, 안정훈1, 최은영1
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| 소속 |
1KSA of KAIST, 2안동대 |
| 키워드 |
MOF; QCM; GFP; His-tag
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| E-Mail |
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