| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 |
9권 2호, p.2092 |
| 발표분야 |
생물화공 |
| 제목 |
Protein profile with benzophenanthridine alkaloids concentration of the Eschscholtzia californica |
| 초록 |
Benzophenanthridine alkaloids, a subclass of the benzylisoquinolines, are produced in an Eschscholtzia californica (California poppy, used as a sedative by Native Americans). These alkaloids concentration increased with carbon source content in culture medium, suggesting that carbon source concentration is important factor for biosynthesis of benzophenanthridine alkaloids. We have performed proteomic analysis of proteins sequentially extracted from E. californica suspended cell which was cultured with various carbon source concentrations and separated by two-dimensional electrophoresis (2-DE). For the soluble proteins in the whole cell, the 2-D separation showed around 2,500 spots, detected under the conditions used in our experiments. And, we found 29 spots of which expression varied analogously with dihydrosanguinarine production. These results demonstrate the use of metabolite analysis as a tool for detecting target proteins related to metabolites production pathway. This study describes the first approach of soluble proteins profiling of E. californica cell culture. |
| 저자 |
박정진1, 윤성용1, 김동수2, 손석영1, 조화영1, 박종문1
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| 소속 |
1포항공과대, 2㈜제노마인 |
| 키워드 |
benzophenanthridine alkaloids; Eschscholtzia californica; proteomics
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| E-Mail |
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| 원문파일 |
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