| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 |
10권 1호, p.390 |
| 발표분야 |
생물화공 |
| 제목 |
Transcriptional profiling and benzylisoquinoline Alkaloids Production of CYP82B1 in Eschscholtzia californica |
| 초록 |
The California Poppy (Eschscholtzia californica) is an ancient medicinal plant capable of producing several benzylisoquinoline alkaloids of pharmaceutical importance, including the antibiotic sanguinarine and chelerythrine, protein kinase C inhibitor. Benzylisoquinoline alkaloids biosynthesis begins with the conversion of L-tyrosine to dopamine and comprises several biosynthetic steps to sanguinarine. The gene of (S)-N-methylcoclaurine-3’-hydroxylase (CYP82B1; Huang and Kutchan, 2000) involved in benzylisoquinoline alkaloid biosynthesis has been isolated from the California Poppy. We constructed the probe of CYP82B1 by PCR and studied CYP80B1 gene transcription level with elicitation. We also investigated several metabolites production level after biotic elicitation by HPLC analysis in our suspended cells. It gave us the relationship between transcription level and metabolite production level, which can be expected to offer the critical information of the bottleneck in sanguinarine production pathway. Our data will provide insight into the complex regulation of benzylisoquinoline alkaloids biosynthesis. |
| 저자 |
손석영1, 윤성용1, 최윤희2, 정호승1, 박정진1, 박종문1
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| 소속 |
1포항공과대, 2제노마인(주) |
| 키워드 |
benzylisoquinoline alkaloids; CYP82B1; eschschotlzia californica
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
