| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Elucidation of Two Parallel post-PKS steps in FK506 Biosynthetic Pathway. |
| 초록 |
FK506 is 23-memebered macrocyclic polyketide of microbial origin exhibiting various pharmaceutical activities. The formation of FK506 requires final post-PKS modification steps, C9-oxidation catalyzed by cytochrome P450 hydroxylase (FkbD), and 31-O-methylation by S-adenosylmethionine (SAM)-dependent methyltransferase (FkbM). Each gene was characterized by in-frame deletion and in vitro enzymatic reactions. The FK506 biosynthetic intermediates involved in post-PKS modification were analyzed by in-depth NMR, HPLC−ESI-MS/MS, and high-resolution MS (HR-MS) data. This study demonstrates two parallel pathways of post-PKS modification using the 9-deoxo-31-O-demethyl-FK506 as a starter in FK506 biosynthesis. And it has been shown that 9-deoxo FK506 derivatives can be used as substrates for FkbD, whereas FkbM enzyme can use 31-O-demethyl derivatives. These two post-PKS modification enzymes can provide a potential tool for the combinatorial biosynthesis of novel derivatives. |
| 저자 |
김희진, 황재연, 김면수, 정진아, 조항수, 윤여준
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| 소속 |
이화여자대 |
| 키워드 |
Streptomyces sp. KTCT11604BP; FK506 analogue; Post-modification
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| E-Mail |
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