| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
고분자가공/복합재료－나노카본 고분자 복합필름 |
| 제목 |
3D on-chip flow-through polymerase chain reaction utilizing a single heater |
| 초록 |
Two poly(dimethylsiloxane) (PDMS) substrates patterned with microchannels were vertically stacked to realize a 3D fluidic conduit and the assembled microdevice was used for performing on-chip continuous-flow polymerase chain reaction (PCR). The assembled two PDMS substrates were bonded with a glass substrate and placed atop a heater controlled at the denaturation temperature of the nucleic acid. When the assembled microdevice is placed atop a heater with the glass-side down, the interface of two PDMS assembly displayed relatively lower temperature than that of the PDMS-glass assembly, as temperature gradient is formed in vertical direction due to the low thermal conductivity of the PDMS. A desired annealing/extension temperature was precisely controlled by molding the bottom PDMS layer to an appropriate thickness. In this way, multiple heating sources were replaced with a single heater, enhancing the device portability. |
| 저자 |
이내윤1, 오문명2, Kieu The Loan Trinh1
|
| 소속 |
1경원대, 2한국생명공학(연) |
| 키워드 |
poly(dimethylsiloxane); microdevice; polymerase chain reaction; micropillar array
|
| E-Mail |
|
