| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1753 |
| 발표분야 |
생물화공 |
| 제목 |
Development of an Open Sandwich Fluoroimmunoassay Based on FRET |
| 초록 |
We have developed a sensitive, one-step, homogeneous open sandwich fluoroimmunoassay (OsFIA) based on fluorescence resonance energy transfer (FRET) and luminescent semiconductor quantum dots (QDs). In this FRET assay, estrogen receptor-β (ER-β) antigen was incubated with QD-labeled anti-ER-β monoclonal antibody and AF-labeled anti-ER polyclonal antibody for 30 minutes, followed by FRET measurement. The dye separation distance was estimated to be between 80-90 Å. The present method is rapid, simple and highly sensitive, and did not require the bound/ free reagent separation steps and solid-phase carriers. A concentration as low as 0.05 nM (2.65 ng/ml) receptor was detected with linearity. In addition, the assay was performed with commercial antibodies. This assay provides a convenient alternative to conventional, laborious sandwich immunoassays. |
| 저자 |
이문권1, 이은규1, Quande Wei1, 주재범2, 성기훈3
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| 소속 |
1한양대, 2Sun Yat-sen Univ., 3China |
| 키워드 |
FRET; Sandwich Immunoassay; Quantum Dots; Estrogen Receptor B
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
