| 학회 | 한국공업화학회 |
| 학술대회 | 2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 23권 2호 |
| 발표분야 | 포스터_생물공학 |
| 제목 | GABA Production from glucose in E.coli with 13C MFA |
| 초록 | We engineered E.coli to synthesize GABA through knocking out byproduct pathway related genes and overexpressing modified glutamate decarboxylase which can synthesize GABA under neutral pH condition. However, as the titer of GABA was still low, 13C MFA was conducted to determine metabolic flux ratios of central carbon metabolism to identify metabolic bottleneck for GABA production. From 13C MFA result, intracellular metabolic flux ratios were successively quantified and rational metabolic engineering targets were determined. We enhanced anaplerotic flux by introducing heterologous pyruvate carboxylase from C. glutamicum and additionally down regulated TCA flux by knocking out sucA to reduce byproduct secretion by succinate. Finally, 13C MFA was carried out to verify optimal central carbon metabolic flux ratios for GABA production. Engineered E.coli strain synthesize 2 g/L of GABA in defined medium condition. This is the first report on GABA synthesis from defined medium condition. |
| 저자 | 이보영, 임대균, 오민규 |
| 소속 | 고려대 |
| 키워드 | GABA; 13C MFA; TCA cycle |
