| 초록 |
Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade extracellular matrix proteins. They have a key role in processes of embryogenesis, morphogenesis, tissue development. The MMPs are also known that they have an essential role in fundamental cellular processes of proliferation, survival, apoptosis, and cell differentiation. A recent study reported that MMP-2 activity of bone marrow-derived stem cells is reduced during osteogenic differentiation and MMP-13 activity of adipose-derived stem cells is increased during bone formation. In this study, we investigated the proliferation and the MMP activity during osteogenic differentiation of hASCs on Type 1 collagen (Col1) that is due to a major component of matrix proteins in bone tissue. The proliferation is measured using Cell Counting Kit-8 and the MMT activity is determined by Real-Time PCR and zymography. |
