| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
대학원생 구두발표 (영어발표, 발표15분) |
| 제목 |
Development of lipid layered biodegradable polymeric nanoparticle for delivery of bioactive molecules |
| 초록 |
In this study, we synthesize protamine incorporated cationic lipid layered PLGA hybrid nanoparticles (LPHNPs) as a biomolecule delivery system by simple emulsion solvent evaporation method and examined the influence of cationic lipid concentration on the physiochemical properties of LPHNPs. Photon correlation spectroscopy results confirmed relatively monodisperse cationic LPHNPs with a Z-average size of 153 nm and low polydispersity (PDI-0.07) and electron microscopical (TEM) result confirmed the structure of a hydrophobic PLGA core and cationic lipid shell. The surface charge of LPHNPs shifted from a negative value (-24 mV) to a positive value (+60) with the incorporation of cationic lipid on the surface of PLGA. In all concentrations, LPHNPs/DNA complexes exhibited strong transfection efficiency while maintaining high cell viability, suggesting LPHNPs as an excellent and versatile gene delivery system |
| 저자 |
Rajendran Jagathesh chandra bose1, 박한수1, 이수홍2, Arai Yoshie2, 고경원2, 김병주2, 최보규2
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| 소속 |
1중앙대, 2차의과대 |
| 키워드 |
nanoparticles; lipid; gene delivery
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| E-Mail |
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