| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Preparation of “Open/closed” pores of PLGA-microsphere for controlled release of protein drug |
| 초록 |
Poly(D,L-lactic-co-glycolic acid) has been extensively used as a controlled release carrier for drug delivery due to its good biocompatibility, biodegradability, and mechanical strength. Porous PLGA microspheres were fabricated by a emulsion-solvent evaporation technique using Pluronic F127 as an extractable porogen and loaded with protein (lysozyme) by suspending them in protein solution. For controlled release of protein, porous microspheres containing lysozyme were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. The surface morphology of microspheres were investigated using scanning electron microscopy (SEM) for confirmation of its porous structure. Structural integrity of the lysozyme were observed by enzymatic activity assay, size-exclusion chromatography (SEC), gel electrophoresis (SDS-PAGE.). |
| 저자 |
오돈치메1, 김성철2, 강수용2, 박아름2, 이우경2
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| 소속 |
1Inje Univ. department of Nano-system Engineering, 2인제대 |
| 키워드 |
PLGA-microsphere; porous microspheres; lysozyme
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| E-Mail |
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