| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.441 |
| 발표분야 | 생물화공 |
| 제목 | Control of Antibody Immobilization Based on Flow Dynamic Self-assembly |
| 초록 | Proper choice of antibody immobilization method is one of the important points in the fabrication of bioactive thin film for immunosensor and protein array. Molecular thin film based on self-assembly (SA) technique is a versatile and in vitro biosurface which mimics the naturally recognition process due to reliable control of density and environment of an immobilized recognition centre. In this study, flow dynamic self-assembly (FDSA) is used for antibody immobilization. Spray device designed in our laboratory spouts antibody fragments solution, resulting in the immobilization of antibody fragments onto the gold surface via their native thiol group (-SH). The fragment of monoclonal antibody (Mab) against human serum albumin (HSA) was prepared and the deposition on the surface was investigated using surface plasmon resonance spectroscopy (MultiskopTM, Optrel GBR, German). Topographies of antibody fragments film was analyzed using atomic force microscopy (CP, PSI, USA). From the experimental result, the amount of antibody-antigen binding was changed by sprayed immobilization method. * 본 연구는 환경부 차세대 핵심 환경기술개발사업의 일환으로 수행되었습니다. |
| 저자 | 이두봉, 이우창, 천범석, 이원홍, 최정우 |
| 소속 | 서강대 |
| 키워드 | antibody immobilization; flow dynamic self-assembly; surface plasmon resonance |
| 원문파일 | 초록 보기 |
