| 초록 |
Nanoparticles of the complex between glabridin and chitosan were spontaneously formed by adding glabridin into chitosan or three N-acylated chitosans which were synthesized by reacting chitosan with acid anhydride. N-acylated chitosans were characterized by FT-IR and 1H-NMR. Degrees of N-acyl substitution of N-propionic, N-butanoic, and N-hexanoic chitosan were 28.2, 34.74, and 35.02 %, respectively. TEM showed the morphology of the complex particles was nano-scale spherical shape, and average size of the particles estimated by particle analyzer was between 32 and 42 nm. Maximum encapsulation efficiency of glabridin was found to be 84% when N-propionic chitosan with 1% glabridin was used for the preparation of nanoparticles, and the efficiency decreased upon increasing glabridin concentration. Stability of glabridin under UV-ray was enhanced after the complex formation with chitosan and N-acylated chitosan. Long-term stability of glabridin in aqueous solution was also affected by the complex formation, and 2.2-fold stability enhancement at 25℃ after 7-day incubation was observed. |
