| 초록 |
Microalgae is photosynthesis unicellular organisms. Arthrospira platensis (A. platensis) belongs to cyanobacterium and culture in tropic environment. A. platensis consists of 60-70% protein, 15-20% carbohydrate and 6-9% lipid. In this study, we were treated to A. platensis with physical and chemical mutagen for order to A. platensis mutant. Commonly, physical mutagen is electron-beam, ultra-violet(UV) and X-ray. Chemical mutagen was known as ethyl methane sulfonate(EMS), N-nitro-N-methyl urea(NMU) and diethyl sulfite(DES). We were obtained to mutants that induced by electron-beam, UV and NMU. When mutants were cultivated to 12-days, we were measured to cell growth rate, protein content, dry cell weight and pigment, lipid content. As a result, cell growth rate of NMU2-5 was increased to 1.9-fold compared to wild type(WT). Protein content and dry cell weight of mutants were similar with WT. Pigment(chlorophyll and carotenoid) content of UV1-2 and NMU 2-5 were increased to 2.5-fold compared to WT. Lipid content of UV1-2 was increased to 1.4-fold compared to WT. |
