| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 가을 (10/22 ~ 10/24, 대전 DCC) |
| 권호 |
20권 2호, p.1615 |
| 발표분야 |
생물화공 |
| 제목 |
DNA immobilized probe assay for helicobacter pylori gastric cancer DNA고정센서의 위암 원인균 진단 |
| 초록 |
Helicobacter pylori infection is related to the most common gastric cancer infection of the world population. The diagnostics of such assay mainly depends on PCR amplification, electric separation, and DNA amplifications by expensive methods. However, DNA immobilized voltammetric probe has a simplified advantage of being fast, economically sensible, and does not require additional equipment or experience. Which new real time probe redox reaction is based on a cyclic and stripping or chrono techniques, working probe was developed for the detection of Helico DNA standard, in vivo injected tissues from animal models and clinical samples performed. The diagnostics showed high analytic statistics (r2 ＞ 1.09) and specificity for the standard addition methods (98%); results of stripping attained to the lower limit of detection was mg ranges of DNA/μL reaction. Which method can be usable for diagnostic real assay for human organs. |
| 저자 |
김대홍1, 이 경2, 장유섭3, 권 담3, 이준민4, 박병현4, 박진우4, 유경우4, 이수영5
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| 소속 |
1Cornell Univ., 2Fudan Univ., 3숙명여고, 4대진고, 5서울과학기술대 |
| 키워드 |
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| E-Mail |
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| 원문파일 |
초록 보기 |
