| 초록 |
Previously, we studied photo-crosslinked biodegradable hydrogles based on acrylated Pluronic F-127 tri-block copolymer. The present study aims at the fabrication of biodegradable hydrogel for local sustained drug delivery of angiogenic growth factors such as basic FGF. Biodegradable hydrogels consisting of Pluronic and heparin were prepared by a photo-polymerization method. In order to facilitate entrapment of bFGF into the hydrogels and to sustained release of this growth factor, vinyl conjugated heparin was blended with di-acrylated Pluronic. As a result, a semi-IPN structure of heparin and Pluronic was formed in the hydrogel network. Release rates of basic FGF from the Pluronic hydrogels and Pluronic/heparin blend hydrogels were comparatively examined. When bFGF was loaded in the Pluronic hydrogels, the presence of heparin modulated initial burst of bFGF from the hydrogels Sustained release of bFGF from the hydrogels could be achieved over 10 days. The released fractions of bFGF from Pluronic/heparin blend hydrogels retained its bioactivity for stimulation of cell proliferation of HUVECs during the incubation period. |
