| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.982 |
| 발표분야 |
재료 |
| 제목 |
Mass analysis of neuropeptides in salty environment using hydrophilic ring-shaped anchors |
| 초록 |
For more than a decade, researchers have studied cell-to-cell signaling peptides using various analytical methods. Signaling peptides have been known to have important role in the physiological mechanisms. Currently available approaches for analyzing peptides have drawbacks such as loss of peptides and complicated steps for sample preparation. We developed an efficient platform for mass analysis of neuropeptides that simultaneously concentrates and desalts the analytes via coffee ring effect. Our approach is to use C18-Au coated patterned substrate. When peptide solution with high salt content is dropped onto the patterned C18-Au surrounded by donut-shaped hydrophilic anchors, salts are removed by outward flow generated during evaporation, while peptides are collected onto the central C18-Au region. Our approach greatly simplifies the sample preparation for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We have developed a simple kinetic model that describes the correlation between the evaporation rate and the surface coverage of peptides. |
| 저자 |
윤 숙, Anar Zhexembekova, 이창영
|
| 소속 |
UNIST |
| 키워드 |
화공소재 전반 |
| E-Mail |
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| 원문파일 |
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