| 초록 |
The spread of bacteria and antimicrobial resistance is becoming a serious threat to public health. It is crucial not only to identify the pathogen, but also the specific type of resistance, for subsequent treatment of the patient. Conventional methods for diagnosing infections have relied on culture-based assays which involve time consuming procedures and low sensitivity. The recent advenced molecular techniques such as qPCR allow specific detection of targets within hours, but confront challenges such as high costs and lack of specificity. Here, we propose a molecular labeling approach based on nanoparticle detection that can be efficiently applied for detecting antibiotics resistance. Nanoparticle-DNA probes targeting an antibiotics resistance gene, was designed to detect single-strand DNA products obtained by asymmetric amplification. This molecular sensing strategy based on nanoparticle detection will introduce simple and rapid platforms with exceptionally high sensitivity. |
