| 초록 |
Diagnosis of infectious diseases requires a simple and facile assay to detect target analytes by visualization using minimal technical requirements. Herein, we introduce a simple and wash-free assay based on precipitation of fluorescent carbon-dot nanoparticles and amplified target DNA, for detecting multidrug-resistant bacteria. Polyethyleneimine carbon-dots were added with various amounts of DNA with different sizes, resulting in precipitation of the DNA by charge interaction with the carbon-dots and allowing simple visualization under a UV illuminator. The interaction of carbon-dots with DNA was characterized by measuring hydrodynamic sizes using dynamic light scattering and quantitative fluorescence intensity measurements. Finally, the assay could be applied for detecting bacteria-derived DNA, which was obtained from reverse transcription and amplification of the mRNA of the drug-resistance gene KPC from multidrug-resistant Klebsiella pneumoniae. |
