| 초록 |
A simple and facile method for sensing of nucleic acids is in great need for disease biomarker detection and diagnosis. Herein, we introduce a fluorescent nanosensor utilizing carbon dot nanoparticles that form visible precipitates in the presence of target DNA. Polyethylenimine carbon dots were emitted strong photoluminescence and could form precipitates when added with target DNA oligonucleotides. The precipitates could be easily visualized by UV illumination, and data could be acquired as images using a smart phone which were analyzed for quantification. This carbon dot-based assay was validated by varying the amounts and sizes of synthetic DNA, in different buffer conditions. Finally, the assay could be applied as a nanosensor platform for detecting bacterial DNA for the antibiotic resistance gene KPC-2 from Klebsiella pneumoniae. |
