| 초록 |
Complex media containing free amino acids originating from peptone or yeast extract are often used to improve the productivity in recombinant protein production processes. This occurs because L-amino acids for E.coli work as either ‘energy amino acids’ which are catabolised to provide energy, or ‘protein amino acids’ which are incorporated into protein. To predict results of recombinant protein production processes E.coli, supplying amino acids with in silico experiment, it is required to obtain a lot of metabolic data about the E.coli metabolome when the amino acid is supplied. A lot of basic data are needed because the metabolism of E.coli changes in media containing amino acid. To obtain the data about amino acid metabolism of E.coli, we carried out batch fermentation of E.coli W3110 having a selected single amino acid in medium. 10 amino acids-His, Ser, Trp, Phe, Tyr, Ala, Val, Leu, Asp, Glu- were respectively supplied. We analyzed organic acids and amino acids excreted to medium quantitatively. And after hydrolyzing total proteins of the cell, we measured total amino acid of proteins constructing the cell in respective experiments. |
