| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.257 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Preparation of Glycosylated poly(4-vinyl imidazole) as a gene carrier |
| 초록 |
Recently reported poly(4-vinyl imidazole)(P4V) is a cationic polymer which has high transfection efficiency with low cytotoxicity owing to pH-responsive endosomolytic escape. But P4V is insoluble in aqueous solution above at pH 6. To overcome this drawback, we modified P4V with gluconic acid (GA-P4V) to impart solubility at physiologicical pH. The GA-P4V was synthesized by amidation of P4V with gluconic acid by using an 1-ethyl-3-(3-dimethylaminopropyl) (EDAC)/ N- hydroxysuccinimide (NHS) condensation system. The obtained GA-P4V was characterized by H1-NMR spectroscopy. The GA-P4V/DNA complex formation was confirmed by electrophoresis on 1.0 % agarose gel. Particle sizes were determined using a light scattering spectrophotometer (ELS 8000, Otsuka Electronics, Ltd., Osaka, Japan). Cytotoxicity test was used by Cell Proliferation Assay Kit (Promega Co.) with several cells. The in vitro transfection experiment will be checked by luciferase assay. The GA-P4V will be expected to be useful for clinical application of gene therapy with improving the transfection efficiency and cell viability.
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| 저자 |
박미란1, 임종은1, 한기옥2, 안광덕3, 조종수1
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| 소속 |
1서울대, 2성균관대 의대 삼성제일병원 내분비내과, 3한국과학기술(연) |
| 키워드 |
gene delivery; poly(4-vinyl imidazole); gluconic acid
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| E-Mail |
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