| 초록 |
3D porous chitosan scaffolds for tissue engineering applications were fabricated via liquid-liquid phase or liquid-solid phase separation. The properties of the porous chitosan scaffolds, such as the microstructure, chemical release, water absorption properties and cell proliferation tests were studied and compared with those of conventional scaffolds. Chitosan scaffolds of over 70% of porosity were obtained and the morphology of the pores located on the surface and within the porous scaffolds showed homogenous open pores and excellent interconnectivity. The main pores (50-100 μm) and minute pores (4-10 μm) were clearly observed as the PVP content increased. Also, the porous scaffold showed improved efficiency for cell adhesion after cell culture for 4 h. After 72 h, the cultured cells presented an increased cell proliferation on the porous scaffolds. These results strongly suggest that the porous chitosan scaffolds can be widely used for tissue engineering, including bio-patch and artificial skin applications. |
