| 초록 |
Herein, we have engineered E. coli cells expressing L-tyrosine converting enzymes of tyrosine ammonia lyase (TAL), p-coumarate 3-hydroxylase (C3H), feruloyl-CoA synthase (FCS), and enoyl-CoA hydratase/aldolase (ECH). Genetic circuit of protocatechualdehde and p-hydroxybenzaldehyde production pathways was reconstituted through combinatorial expressions of those enzymes. Firstly, TAL and C3H were coexpressed with FCS and ECH and this strain could convert L-tyrosine into caffeic acid, which was converted into protocatechualdehyde continuously. And the production yields of protocatehcualdehyde and p-hydroxybenzaldehyde by this reconstituted strain reached up to 42.0 mg/L and 133 mg/L, respectively. Especially ascorbic acid was used as a protocatechualdehyde/caffeic acid-based melanin formation inhibitor. Secondly, L-tyrosine was converted into p-coumaric acid by TAL and the p-coumaric acid was converted into p-hydroxybenzaldehyde with 41.0 mg/L of yield by FCS and ECH simultaneously. |
