| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.449 |
| 발표분야 |
생물화공 |
| 제목 |
Engineering the Chimeric Two-Component System in Escherichia coli from Paracoccus denitrificans to sense methanol |
| 초록 |
Abstract Escherichia coli does not have the methanol sensing apparatus, was engineered to sense methanol by employing chimeric two-component system (TCS) strategy. A chimeric FlhS/EnvZ (FlhSZ) chimeric histidine kinase (HK) was constructed by fusing the sensing domain of Paracoccus denitrificans FlhS with the catalytic domain of E. coli EnvZ. The constructed chimeric TCS FlhSZ/OmpR could sense methanol by the expression of ompC and gfp gene regulated by ompC promoter. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed the dynamic response of the chimeric TCS to methanol. The expression of ompC and the gfp fluorescence was maximum at 0.01% and 0.5% of methanol respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric HK FlhSZ. This strategy can be employed for the construction of several chimeric TCS based bacterial biosensors for the development of biochemical producing recombinant microorganisms. This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ01111601) by RDA. |
| 저자 |
홍순호, 정재훈, Vidhya Selvamani, 장현진, 권태희, 유익근
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| 소속 |
울산대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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