| 초록 |
Using SP6 promoter-RNA polymerase as a model, different LSPR λmax shift were recorded with diverse promoter sequences induced by point mutation. In the initial stage of gene transcription RNA polymerase selectively interacts with the promoter site of the gene and form a complex, which is able to initiate RNA synthesis. The degree of gene expression is affected by promoter sequence controls the binding and initiating efficiency of polymerase. An λmax shift will occur in each step and we expect the delta maxs to be different according to the dissimilar types of p53. As a results, between the promoter and p53 proteins affinity difference. The case of wild type, the mutation type is higher than the binding affinity. Also depending on the mutation, affinity appears differently. This result successfully explain a non-labeling detection system for biomolecular interaction and it possesses a superior potential as a sensitive, on-chip and multiplexing detection. |
