| 초록 |
Superparamagnetic iron oxide nanoparticles (SPIO) nanoparticles can be used as magnetic resonance (MR) imaging agents in diagnosis and therapy. A commercially available T2 contrast agent, dextran-SPIO (Feridex®), has been widely applied to cellular labeling. However, Feridex® requires long incubation times, generally over 24 hrs, to be efficiently internalized into cells. The purpose of this study was to develop highly efficient MR T2 contrast agent based on anticoagulant heparin polysaccharide. Two types of biocompatible magnetic agent probes were developed using unfractionated heparin (UFH) and low-molecular-weight-heparin (LMWH); that are called to UFH-SPIO and LMWH-SPIO, respectively. The newly developed SPIOs with high signal intensity, stability, biocompatibility and magnetization can be efficiently internalized into Ins-1 cells, without changing viability and function. |
