| 초록 |
Nitric oxide(NO) has been an interesting signalling molecule to regulate paramount physiological processes, such as the control of vasodilation, immune responses, the inhibition of platelet adhesion and aggregation, angiogenesis, apoptosis, wound healing, tissue repair, neurotransmission and inflammation. In this study, we report feasibility of mPEG-PLGA nanoparticles as nitric oxide releasing polymer. The copolymer was synthesized by ring-opening polymerization of lactide, glycolide and mPEG as a macro-initiator. Double emulsion method was used to prepare mPEG-PLGA nanoparticles incorporating NONOate. The liposomal nanoparticles can effectively encapsulate hydrophilic DETA/NONOate. The nanoparticles with different molecular weight of PEG released NO for varying time respectively and was not cytotoxic in various cell types, including fibroblasts, HUVEC and epithelial cells. Angiogenic potential of the nanoparticles was confirmed in vitro by tube formation and ex vivo aortic assay. |
