| 초록 |
Liposomes used as one of the efficient drug carriers have some shortcomings such as their short circulation time and fast clearance from human body by reticuloendothelial system (RES). In this study, PEG-coated liposomes were prepared by complexation of positively charged liposomes with carboxylated PEG (mPEG-COOH). To confirm incorporation of PEG on the liposomal surface, PEG amount was quantified by picrate assay method. The intracellular uptake of PEG-coated liposomes was evaluated by FACS. The PEG-coated liposomes had 120±2 nm of mean particle size and 12.9±2 mV of zeta potential value. Loading efficiency of model drug, doxorubicin, into PEG-coated liposomes was about 95±2 %. Intracellular uptake of the PEG-coated liposomes was much higher than StealthTM liposomes. The PEG-coated liposomes may be applicable as anticancer drug carriers that can increase circulation time and therapeutic efficacy. |
